Measuring binding kinetics of surface-bound molecules using the surface plasmon resonance technique

Lipid membrane-binding properties of daptomycin using surface plasmon resonance.

In the present study, we developed an assay of interactions of daptomycin with four model lipid membranes that mimicked mammal cell membranes and gram-positive and negative bacteria membranes. We also analyzed the binding kinetics of the gram-positive bacteria membranes using surface plasmon resonance (SPR). Daptomycin showed a higher affinity for the model gram-positive bacteria membrane than ...

Determination of ribonuclease H surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy.

The kinetics of the ribonuclease H (RNase H) surface hydrolysis of RNA-DNA heteroduplexes formed on DNA microarrays was studied using a combination of real-time surface plasmon resonance imaging (SPRI) and surface plasmon fluorescence spectroscopy (SPFS). Time-dependent SPRI and SPFS data at various enzyme concentrations were quantitatively analyzed using a simple model that couples diffusion, ...

Surface plasmon resonance biosensor

Imaging surface plasmon resonance

The central theme of this thesis is the use of imaging Surface Plasmon Resonance (iSPR) as a tool in the characterization of surfaces with laterally varying properties. Within the scope of this work, an instrument for iSPR analysis was designed and built. SPR is a very sensitive technique for monitoring changes in optical properties in the immediate vicinity of a sensor surface, which is very u...

Surface plasmon resonance

4. LIGAND 10 4.1. Direct versus indirect immobilisation 10 4.2. Covalent immobilisation 11 4.2.1. A general approach 11 4.2.2. Choice of chemistry 12 4.2.3. Prepare the protein 12 4.2.4. Pre-concentration 12 4.2.5. Amine coupling 14 4.2.6. Regeneration 15 4.2.7. Adjusting the immobilisation conditions. 16 4.3. Non-covalent immobilisation (ligand capture) 16 4.3.1. Using an existing strategy 17 ...